Cbs-scientific SG Series Uživatelský manuál

Adjustable Height Nucleic Acid Sequencing SystemsINSTRUCTION MANUAL Single Units Dual UnitsSG-400-20 DASG-400-20SG-500-20 DASG-5

C.B.S.  Scientific 10 Adjustable Height Sequencer 3.3 Glass Plate Preparation 1. Select a pair of glass plates (if using a notched set, one n

C.B.S.  Scientific 11 Adjustable Height Sequencer 3.4 Methods for sealing glass plate sandwich (A) Gel Slider, ( B) Capillary Gel Casting or

C.B.S.  Scientific 12 Adjustable Height Sequencer 3.4 Methods for sealing glass plate sandwich - continued (A) Gel Slider, ( B) Capillary Ge

C.B.S.  Scientific 13 Adjustable Height Sequencer 3.5 Pouring the Gel 1. Always wear gloves, eye protection and protective clothing. 2. Pre

C.B.S.  Scientific 14 Adjustable Height Sequencer 6. Before the acrylamide polymerizes, insert a comb into the top of the gel. The available ty

C.B.S.  Scientific 15 Adjustable Height Sequencer Section 4 Electrophoresis 4.1 Pre-electrophoresis 1. Remove the gel sealing tape from the bo

C.B.S.  Scientific 16 Adjustable Height Sequencer 5. Verify that the upper buffer reservoir drain is secure. For the single 20cm wide sequencer

C.B.S.  Scientific 17 Adjustable Height Sequencer 4.3 Electrophoresis 1. After loading the samples, attach the reservoir safety cover. Connect

C.B.S.  Scientific 18 Adjustable Height Sequencer 4.4 Post-Electrophoresis – Gel Processing A. Removal of sequencing plate from gel unit.

C.B.S.  Scientific 19 Adjustable Height Sequencer B. Transfer of gel onto blotting paper-continued 2. Place a sheet of pre-cut (slightly larg

C.B.S.  Scientific 2 Adjustable Height Sequencer TABLE OF CONTENTS Page Important User Information 3-4 Section 1 General Information

C.B.S.  Scientific 20 Adjustable Height Sequencer 4.5 Recommended Buffers and Reagents Pre-mixed acrylamide stock solutions are the method of ch

C.B.S.  Scientific 21 Adjustable Height Sequencer 4.6 References and Suggested Reference Literature DNA SEQUENCING PROTOCOLS in Methods in Mole

C.B.S.  Scientific 22 Adjustable Height Sequencer Section 5 Tables: Power Settings, Troubleshooting Guide, Comb Volumes 5.1 Recommended Powe

C.B.S.  Scientific 23 Adjustable Height Sequencer 5.2 Troubleshooting Guide Problem/Condition Cause & Solution ELECTRICAL  'No Lo

C.B.S.  Scientific 24 Adjustable Height Sequencer 5.3 Comb Volumes Rectangular Combs for 20cm wide units # of wells Tooth width/mm 0.2mm thic

C.B.S.  Scientific 25 Adjustable Height Sequencer 5.3 Comb/Well Maximum Loading Volumes-continued SharktoothCombs for 20cm wide units Note: T

C.B.S.  Scientific 26 Adjustable Height Sequencer 5.3 Comb/Well Maximum Loading Volumes-continued Preparative Combs for 20cm wide units Note

C.B.S.  Scientific 27 Adjustable Height Sequencer Section 6 Maintenance of Equipment 6.1 Care and Handling The components of the Sequencer are f

C.B.S.  Scientific 28 Adjustable Height Sequencer Section 7 Equipment and Accessories ADJUSTABLE NUCLEIC ACID SEQUENCERS Cat.# Item

C.B.S.  Scientific 29 Adjustable Height Sequencer SEQUENCING ACCESSORIES RECTANGULAR COMBS (POLYCARBONATE) 20CM WIDE UNITS 33CM WIDE UNI

C.B.S.  Scientific 3 Adjustable Height Sequencer IMPORTANT USER INFORMATION This Instruction Manual will explain how to use this product safely a

C.B.S.  Scientific 30 Adjustable Height Sequencer PREPARATIVE RECTANGULAR COMBS (TEFLON) 20CM WIDE UNITS Cat.# Comb Dimensions SG20-0701 0.75

C.B.S.  Scientific 31 Adjustable Height Sequencer REPLACEMENT SAFETY COVERS 20CM WIDE UNITS 33CM WIDE UNITS Cat.# Description

www.cbsscientific.com 32 Adjustable Height Sequencer MICROTROUGH CASTING COMBS 20CM WIDE UNITS 33CM WIDE UNITS Cat.# Thickness x # tro

C.B.S.  Scientific 33 Adjustable Height Sequencer SPACER PAIRS BOTTOM SPACERS FOR 20CM & 33CM WIDE UNITS FOR 20CM & 33CM WI

C.B.S.  Scientific 34 Adjustable Height Sequencer WEDGE SPACER SETS FOR 20CM AND 33CM WIDE UNITS EXPONENTIAL 1:3 RATIO EXPONENTIAL 1:5 RATI

C.B.S.  Scientific 35 Adjustable Height Sequencer ULTRATHIN MYLAR ACCESSORIES FOR 20CM & 33CM WIDE UNITS 20CM WIDE UNITS 33CM WIDE

C.B.S.  Scientific 36 Adjustable Height Sequencer ADDITIONAL GUIDE RODS SLIDING GEL CASTERS Cat.# Item Cat.# Item SR-0

C.B.S.  Scientific 4 Adjustable Height Sequencer FRANÇAIS INFORMATION IMPORTANTE À L'USAGE DES UTILISATEURS Le présent manuel d'utilis

C.B.S.  Scientific 5 Adjustable Height Sequencer SECTION 1 General Information 1.1 Introduction The C.B.S. Scientific Adjustable Nucleic Acid S

C.B.S.  Scientific 6 Adjustable Height Sequencer 1.3 Safety Power to the Sequencing unit is to be supplied by an external DC voltage power supp

C.B.S.  Scientific 7 Adjustable Height Sequencer Section 2 Description of Parts 2.1 Unpacking- Adjustable Height Sequencer Please verify th

C.B.S.  Scientific 8 Adjustable Height Sequencer 2.2 Unpacking- Dual Adjustable Height Sequencer Please verify that your sequencing unit comes

C.B.S.  Scientific 9 Adjustable Height Sequencer Section 3 Assembly 3.1 Unit Assembly for Adjustable Height Sequencer 1. Place the Adjustab