Cbs-scientific DGGEK-2401 Uživatelský manuál

Cipher DGGE Electrophoresis SystemsDGGEK-1001 DGGEK-2001DGGEK-2401 INSTRUCTION MANUAL

10Cipher DGGE Instructions 2/28/142.3.4 Controller Operation 2.3.4.a Reservoir Liquid Level Fill the reservoir with the appropriate buffer. Th

11www.cbsscientic.comTo change from °F to °C, place the Circuit Breaker/Power Switch in the OFF position and then press and hold the P3 Button while

12Cipher DGGE Instructions 2/28/14 2.3.4.h Local Lockout FeatureThis feature enables the user to lock all controls on the controller. While

13www.cbsscientic.com2.3.6 Controller Calibrations and Maintenance 2.3.6.a Calibration Calibration allows the user to match the Controller’s

14Cipher DGGE Instructions 2/28/14DGGEK Unit Set-Up, con’t.2.4 Setting Buffer Temperature Warning: Do NOT turn on Heater/Stirrer until tank has

15www.cbsscientic.comTo mains (wall outlet)To mains (wall outlet)Figure 2-2abcdPower Supply Connection

16Cipher DGGE Instructions 2/28/14SECTION 3Gel Casting Options3.1 Preparation/Cleaning of Glass Plate for Gel CastingHand wash both plates with a hig

17www.cbsscientic.com3. Place the gasketed plate on the lab bench with the tubing side up, and extend the bottom of the plate over the edge of the

18Cipher DGGE Instructions 2/28/143.2 Gel Casting Techniques- continuedB. Vertical gradient gel casting using GM-40 gradient maker and gravity ow.1.

19www.cbsscientic.com3.2 Gel Casting Techniques- continuedC. Vertical gradient gel casting using gradient maker (GM-40) and a mini-pump. 1. Alter

2Cipher DGGE Instructions 2/28/14TABLE OF CONTENTS Important User Information . . . . .

20Cipher DGGE Instructions 2/28/143.2 Gel Casting Techniques- continued D. Vertical gradient gel casting using GM-40 gradient maker, Mini-Pump and

21www.cbsscientic.com3.3 Vertical Gradient Formation To determine the range of gradient appropriate for your fragment analysis, please read the en

22Cipher DGGE Instructions 2/28/14FIGURE 1FIGURE 21.0mm AND THICKER.75mm AND THINNERPERPENDICULAR GEL CASTINGTO GRADIENT MAKER20 GAUGE NEEDLESEAL WITH

23www.cbsscientic.com3.5 Perpendicular Gradient FormationTo determine the range of perpendicular gradient appropriate for your fragment analysis, p

24Cipher DGGE Instructions 2/28/14SECTION 4 Gel Running Preparations4.1 Preparation of the Cassettes1. After polymerization rinse gel plate assembl

25www.cbsscientic.com4.2 Buffer Cycling Connections1. Fill upper reservoir(s) by attaching recirculating tubing to barb tting on each upper reser

26Cipher DGGE Instructions 2/28/144.2 Buffer Cycling Connections-continued.Buffer Cycling Connections – Dual Cassettes SECTION 5Running C

27www.cbsscientic.com5.2 Removing the Gels3. After the run is completed, turn off power supply, disconnect recirculating tubes and power leads, remo

28Cipher DGGE Instructions 2/28/14SECTION 6Recommended Buffers and Reagents Warning: Do not mix buffer in the DGGE tank! 6.1

29www.cbsscientic.comSECTION 7Maximum Well/Comb Volumes Vertical Comb Options, deeper wells, overall length = 13.1cmCat. # Tooth Depth(mm)# of T

3www.cbsscientic.comIMPORTANT USER INFORMATIONThis Instruction Manual will explain how to use this product safely and effectively. Please read and c

30Cipher DGGE Instructions 2/28/14SECTION 8 References1. S.G. Fischer and L.S. Lerman, (1983) PNAS 80:1579.2. R.M. Myers, T. Maniatis, L.S. Lerman,

31www.cbsscientic.comSECTION 9Troubleshooting 9.1 Unit Will Not Operate (no heat, cooling, or pumping) • Check that the power cord is plugged in to

32Cipher DGGE Instructions 2/28/14SECTION 10Maintenance of Equipment10.1 Care and HandlingThe plastic components of the DGGE Systems are fabr

33www.cbsscientic.comSECTION 11Ordering Information CAT. # DESCRIPTIONCIPHER DGGE ELECTROPHORESIS SYSTEMSDGGEK-1001Cipher DGGE Electrophoresis Syst

34Cipher DGGE Instructions 2/28/14 

35www.cbsscientic.com 13R, 712, 7306, 7312, 812, 8006, 8012, 8002, 8112, 8102, 8106, 8202, 8212, 8206, 8306, 9006, 9012, 9002, 912, 9112, 9102, 91

CONTACT INFORMATIONOnline Ordering www.cbsscientic.comTelephone Local and International: 858-755-4959 Toll Free: 800-243-4959Sales E-mail Address

4Cipher DGGE Instructions 2/28/14FRANÇAIS INFORMATION IMPORTANTE À L’USAGE DES UTILISATEURSLe présent manuel d’utilisation explique la manière de se

5www.cbsscientic.comSECTION 1General Information 1.1 IntroductionDenaturing Gradient Gel Electrophoresis (DGGE) is a powerful genetic analysis

6Cipher DGGE Instructions 2/28/14 1.3 SafetyPower to the DGGE systems is to be supplied by an external DC voltage power supply that must be grou

7www.cbsscientic.comFigure 2-1 HeaterStirrerBuffer SiphonPump AssemblyVaporShieldThermometerDGGETankSingleCassetesComb (2

8Cipher DGGE Instructions 2/28/14   Front View Rear View 1. Dis

9www.cbsscientic.com   1. Temperature Sensor 2. Pump Shaft and Impeller 3. Pump Outlet Nozzle1 4. Over-Temperature S